报告题目：Amorpha-4,11-diene synthase, a key enzyme in artemisinin biosynthesis in Artemisia annua
　  报告人：Prof. Erik Peter Brodelius
　　　　　  School of Pure and Applied Natural Sciences, University of Kalmar, Sweden
　  时间：2009年4月14日 14：00
　  地点：实验楼报告厅
　  联系人：刘本叶 (benyel@ibcas.ac.cn)
　
　  报告人简介：
　  Peter Brodelius博士，瑞典Kalmar大学生物化学教授，曾任Kalmar大学Natural Science & Engineering学院院长(1999－2007)。主要研究领域为植物次生代谢途径及其调控，在植物萜类化合物特别是青蒿素生物合成关键酶的基因克隆，酶催化机制分析和青蒿素代谢工程方面做了大量有工作。培养博士后14人，博士研究生10人，已发表研究论文130多篇，2004年，获Delaware House of Representatives (USA) Certificate of Appreciation - for biotechnology奖励。
　  网址：http://www2.hik.se/applikationer/forskningspresentation/researcher.aspx?culture=en&personid=4541
　 
　  Relevant publications:
　  1.M.E. Olsson, L.M. Olofsson, M. Brodelius, P.E. Brodelius (2009) Isolation of whole apical and subapical cells of glandular secretory trichomes of Artemisia annua L. by a modified laser microdissection pressure catapulting technique; Localization of enzymes of artemisinin biosynthesis to the apical cells. Submitted
　  2.S. Picaud, M.E. Olsson, P.E. Brodelius (2007) Improved conditions for production of recombinant plant sesquiterpene synthases in E. coli, Prot. Exp. Purif. 51:71-79
　  3.S. Picaud, P. Mercke, X. He, M. Brodelius, O. Sterner, D. Cane, P.E. Brodelius (2006) Amorpha-4,11-diene synthase. Mechanism and stereochemistry of the enzymatic cyclization of farnesyldiphosphate, Arch. Biochem. Biophys. 448:150-155
　  4.A.-L. Lindahl, M.E. Olsson, P. Mercke, ？. Tollbom, J. Schelin, M. Brodelius and P.E. Brodelius (2006) Expression of amorpha-4,11-diene synthase in yeast. Comparison of transformation by plasmid and homologous recombination. Biotechnol. Lett. 28:571-580
　  5.M. Brodelius, M. Hiraiwa, S. Martilla, S. Picaud, S. Al-Karadaghi and P.E. Brodelius (2005) Immunolocalization of the saposin-like insert of plant aspartic proteinases exhibiting saposin C activity in seeds and young flower tissues. Physiol. Plant. 125:405-418
　  6.S. Picaud, M. Brodelius and P.E. Brodelius (2005) Expression, purification and characterization of ？-farnesene synthase from Artemisia annua L. Phytochemistry 66:961-967
　  7.S. Picaud, L. Olofsson, M. Brodelius and P.E. Brodelius (2005) Expression, purification and characterization of amorpha-4,11-diene synthase from Artemisia annua L. Arch. Biochem. Biophys. 369:213-222
　  8.M. Brodelius, A. Lundgren, P. Mercke and P.E. Brodelius (2002) Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum. Eur. J. Biochem 269:3570-3577
　  9.P. Mercke, M. Bengtsson, H.J. Bouwmeester, M.A. Posthumus and P.E. Brodelius (2000) Cloning, expression and characterization of amorpha-4,11-diene synthase, a key enzyme of artemisinin biosynthesis in Artemisia annua L. Arch. Biochem. Biophys. 381:173-180
　  10.P. Mercke, J. Crock, R. Croteau and P.E. Brodelius (1999) Cloning, expression and characterization of epi-cedrol synthase, a sesquiterpene cyclase from Artemisia annua L. Arch. Biochem. Biophys. 369:213-222

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