姓  名: 王柏臣
职务/职称: 研究员、研究组组长
联系电话: 86-10-62836884
电子邮件: wangbc@ibcas.ac.cn
个人网页: http://www.klpbcas.com/yjdw/ktzz/wbc/
课 题 组: 光合碳代谢研究组
王柏臣,男,博士,研究员,博士生导师。

2002年在哈尔滨师范大学获得硕士学位,2006年在北京大学获博士学位。20069月至20116月在东北林业大学担任教授从事教学和科研工作。以通讯作者和第一作者发表SCI收录论文30余篇。已培养硕士和博士19名;在读博士生和硕士生8名。

主要研究工作:

1 植物光合碳代谢研究:

C4植物玉米和C3植物拟南芥和杨树为研究材料,利用分子生物学,生物化学、细胞学和遗传学方法研究C4植物高效固碳的分子生物学机理以及C4途径关键酶酶转录、翻译、翻译后修饰以及酶活活性的调控机制。

主持和参加的科研项目:

1杨树Pin1At蛋白控制株型发育的分子机制,国家自然基金面上项目;项目批准编号:32371909,(2024.1-2027.12)资助额度:50万元,主持人

2杨树木材形成关键基因的挖掘,科技创新2030-重大项目;编号:2023ZD04069052023.12-2025.12)资助额度:270万元,任务负责人

3基于高附加值生物质基需求的高固碳树种创制,CAS概念验证计划;项目编号:CAS-GNYZ-2022,(2023.7-2024.7)资助额度:20万元,主持人。

4南疆抗逆、高产优质苜蓿品种选育及高效栽培技术研究与示范,兵团科技计划项目;(2023.1-2025.12)资助额度:85万元,主持人。

5苜蓿蛋白表达生物反应器系统的建立和优化,横向课题;项目编号20221100430050262019C004,(2022.5-2025.4)资助额度:200万元,主持人。

6作物高光效利用的分子机制,中国科学院战略性先导科技专项;子课题编号:XDA240102032019.11-2024.12)资助额度:1084万元,子课题负责人。

7杨树和苜蓿等重要林草植物基因编辑技术的建立,横向课题;项目编号:2019C092,(2019.11-2023.10)资助额度:500万元,主持人。

8盐碱地草业发展关键技术集成与示范,中国科学院科技服务网络计划;编号:2016ZX08009003-005-001,(2019.1-2020.6)资助额度:40万元,课题负责人。

9高光效优质牧草品种培育和扩繁技术研发,横向课题;项目编号:2019C004,(2019.1-2023.12)资助额度:100万元,主持人。

10玉米光合固碳途径关键酶PPDK调节蛋白PDRP的双功能调控,国家自然科学基金面上项目;编号:31770262,(2018.1-2021.12)资助额度:60万元,主持人。

11玉米产量、品质性状的功能基因组与调控网络,国家重点基础研究发展计划;课题编号:2016YFD0101003,(2016.7-2021.12)资助额度:60万元,子课题负责人。

12重要性状基因克隆及功能验证,转基因生物新品种培育重大专项;编号:2016ZX08009003-005-001,(2016.1-2020.12)资助额度:135万元,任务负责人。

13玉米PEPCK-C4光合碳代谢途径的调控机制研究,国家自然科学基金面上项目;编号:31471506,(2015.1-2018.12)资助额度:100万元,主持人。

14玉米矮杆基因及其调控网络的发掘,国家自然科学基金重大研究计划培育项目;编号:91435109,(2015.1-2017.12)资助额度:100万元,主持人。

15光高效玉米种植模式增产机理研究,中国科学院科技服务网络计划,项目编号:KFJ-EW-STS-0662014.5-2016.5)资助额度:79万元,课题负责人。

16玉米水分高效利用分子模块解析,中国科学院战略性先导科技专项;子课题编号:XDA080102062013.8-2018.7)资助额度:3089万元,子课题负责人。

17杨树抗旱光合关键基因的鉴定及分子育种技术研究,863项目,批准号:2013AA1027012013.1-2017.12)资助额度:133万元,子课题负责人。

18玉米高光效QTL克隆及分子机理研究,863项目,批准号:2012AA10A3002012.1-2015.12)资助额度:66万元,子课题负责人。

19玉米高光效分子机理研究,中国科学院知识创新工程项目; 批准号:O2819G1001,(2011.1-2013.12)资助额度:200万元,主持人。

20杨树引入C4光合固碳途径关键酶的分子基础研究,国家自然基金重点项目; 批准号:31030017,(2011.1-2014.12)资助额度:204万元,主持人。

研究论文(注*为通讯作者):

2023

  1. Gao ZF,Yang X,Mei YC,Zhang J,Chao Q*,Wang BC*. 2023. A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes. Plant J,113: 291-307.
  2. Yang MY,Yang X,Yan Z,Chao Q,Shen J,Shui GH,Guo PM,Wang BC*. 2023. OsTST1,a key tonoplast sugar transporter from source to sink,plays essential roles in affecting yields and height of rice (Oryza sativa L.). Planta,258(1):4.
  3. Zhang SY,Zhao BG,Shen Z,Mei YC,Li G,Dong FQ,Zhang J,Chao Q*,Wang BC*. 2023. Integrating ATAC‑seq and RNA‑seq to identify differentially expressed genes with chromatin‑accessible changes during photosynthetic establishment in Populus leaves. Plant Mol Biol,113: 59-74.

2022

  1. Yan Z,Yang MY,Zhao BG,Li G,Chao Q,Tian F,Gao G,Wang BC*. 2022. OsAPL controls the nutrient transport systems in the leaf of rice (Oryza sativa L.). Planta,256: 11.
  2. Zhao BG,Li G,Wang YF,Yan Z,Dong FQ,Mei YC,Zeng W,Lu MZ,Li HB,Chao Q*,Wang BC*. 2022. PdeHCA2 affects biomass in Populus by regulating plant architecture,the transition from primary to secondary growth,and photosynthesis. Planta,255: 101.

2020

  1. Yan Z,Shen Z,Chao Q,Kong L,Gao ZF,Li QW,Zheng HY,Zhao CF,Lu CM,Wang YW*,Wang BC*. 2020. Genome-wide transcriptome and proteome profiles indicate an active role of alternative splicing during de-etiolation of maize seedlings. Planta,252: 60.
  2. Gao ZF,Shen Z,Chao Q,Yan Z,Ge XL,Lu TC,Zheng HY,Qian CR*,Wang BC*. 2020. Large-scale proteomic and phosphoproteomic analysis of maize seedling leaves during de-etiolation. Genom Proteom Bioinf,18: 6.
  3. Yan Z,Shen Z,Gao ZF,Chao Q,Qian CR,Zheng HY,Wang BC*. 2020. A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de-etiolation in Zea mays. J Plant Physiol,248: 153-158.

2019

  1. Chao Q,Gao ZF,Zhang D,Zhao BG,Dong FQ,Fu CX,Liu LJ,Wang BC*. 2019. The developmental dynamics of the Populus stem transcriptome. Plant Biotechnol J,17: 206-219.
  2. Wang YF,Chao Q,Li Z,Lu TC,Zheng HY,Zhao CF,Shen Z,Li XH*,Wang BC*. 2019. Large-scale identification and time-course quantification of ubiquitylation events during maize seedling de-etiolation. Genom Proteom Bioinf,17: 603-622.

2017

  1. Li Y,Dong XM.,Jin F,Shen Z,Chao Q,Wang BC. 2017. Histone acetylation modifications affect tissue-dependent expression of poplar homologs of C4 photosynthetic enzyme genes. Front Plant Sci,8: 950.
  2. Li Y,Jin F,Chao Q,Wang BC*. 2017. Proteomics analysis reveals the molecular mechanism underlying the transition from primary to secondary growth of Poplar. J Plant Physiol, 213: 1-15.
  3. Shen Z,Dong XM,Gao ZF,Chao Q,Wang BC. 2017. Phylogenic and phosphorylation regulation difference of phosphoenolpyruvate carboxykinase of C3 and C4 plants. J Plant Physiol,213: 16-22.
  4. Bu TT,Shen J,Chao Q,Shen Z,Yan Z,Zheng HY,Wang BC. 2017. Dynamic N-glycoproteome analysis of maize seedling leaves during de-etiolation using Concanavalin A lectin affinity chromatography and a nano-LC–MS/MS-based iTRAQ approach. Plant Cell Rep,36: 1943-1958.

2016

  1. Jiang L,Chen YB,Zheng JG,Chen ZH,Liu YJ,Tao Y,Wu W,Chen ZZ,Wang BC*. 2016. Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein. Plant Physiol,170: 732-741.
  2. Chao Q,Gao ZF,Wang YF,Li Z,Huang XH,Wang YC,Mei YC,Zhao BG,Li L,Jiang YB,and Wang BC*. 2016. The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins. Plant Mol Biol,91: 287-304.
  3. Dong XM,Li Y,Chao Q,Shen J,Gong XJ,Zhao BG,Wang BC*. 2016. Analysis of gene expression and histone modification between C4 and non-C4 homologous genes of PPDK and PCK in maize. Photosynth Res,129: 71-83.
  4. Chen YB,Wang D,Ge XL,Zhao BG,Wang XC,Wang BC*. 2016. Comparative proteomics of leaves found at different stem positions of maize seedlings. J Plant Physiol,198: 116-28.
  5. Ning DL,Liu KH,Liu CC,Liu JW,Qian CR,Yu Y,Wang YF,Wang YC,Wang BC*. 2016. Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening. Planta,243: 501-507.

2015

  1. Jiang L,Chen YB,Zheng JG,Chen ZH,Liu YJ,Tao Y,Wu W,Chen ZZ,Wang BC*. 2015. Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein (PDRP). Plant Physiol,170(2):732.

2014

  1. Meng LB,Chen YB,Lu TC,Wang YF,Qian CR,Yu Y,Ge XL,Li XH,Wang BC*. 2014. A systematic proteomic analysis of NaCl-stressed germinating maize seeds. Mol Biol Rep,41(5):3431-3443.

2011

  1. Bi YD,Wang HX,Lu TC,Li XH,Shen Z,Chen YB,Wang BC*. 2011.Large-scale analysis of phosphorylated proteins in maize leaf. Planta,233: 383-392.
  2. Liu XY,Wu YD,Shen ZY,Shen Z,Li HH,Yu XM,Yan XF,Guo CH,Wang BC*. 2011.Shotgun proteomics analysis on maize chloroplast thylakoid membrane. Front Biosci,3: 250-5.
  3. Bi YD,Wei ZG,Shen Z,Lu TC,Cheng YX,Wang BC*,Yang CP. 2011. Comparative temporal analyses of the Pinus sylvestris L. var. mongolica litv. apical bud proteome from dormancy to growth. Mol Biol Rep,38: 721-729.

2010及以前

  1. Liu CC,LuTC,Li HH,Wang HX,Liu GF,Ma L,Yang CP,Wang BC*. 2010.Phosphoproteomic identification and phylogenetic analysis of ribosomal P-proteins in Populus dormant terminal buds. Planta,231: 571-581.
  2. Ni RJ,Shen Z,Yang CP,Wu YD,Bi YD,Wang BC*. 2010. Identification of low abundance polyA-binding proteins in Arabidopsis chloroplast using polyA-affinity column.Mol Biol Rep,37: 637-641.
  3. Shen Z,Li P,Ni RJ,Mark R,Yang CP,Liu GF,Ma W,Liu GJ,Ma L,Li SJ,Wei ZG,Wang HX,Wang BC*. 2009.Label-free quantitative proteomics analysis of etiolated maize seedling leaves during greening.Mol Cellular Proteomics,8: 2443-2460.
  4. Zhu H,Bi YD,Yu LJ,Guo DD,Wang BC*. 2009. Comparative proteomic analysis of apomictic monosomic addition line of Beta corolliflora and Beta vulgaris L. in sugar beet.Mol Biol Rep,36: 2093-8.
  5. Lu TC,Meng LB,Yang CP,Liu GF,Liu GJ,Ma W,Wang BC*. 2008. A shotgun phosphoproteomics analysis of embryos in germinated maize seeds.Planta,228: 1029–1041.
  6. Wu FZ,Lu TC,Shen Z,Wang BC*,Wang HX. 2008.N-Terminal acetylation of two major latex proteins from Arabidopsis thaliana using electrospray ionization tandem mass spectrometry.Plant Mol Biol Rep,26: 88-97.
  7. Wang BC,Pan YH,Meng DZ,Zhu YX. 2006.Identification and quantitative analysis of significantly accumulated proteins during the Arabidopsis seedling de-etiolation process. J Integr Plant BioL,48: 104-113.
  8. Wang BC,Wang HX,Feng JX,Meng DZ,Qu LJ,Zhu YX. 2006. Post-translational modifications,but not transcriptional regulation,of major chloroplast RNA-binding proteins are related to Arabidopsis seedling development. Proteomics,6: 2555-2563.