Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis
作 者：Zhang DJ, Wang XM, Wang M, Li JH, Guo XY, Chong K, Xu YY*
影响因子：3.73
刊物名称：PLoS One
出版年份：2013
卷：8 期：9 页码：e75773
论文摘要：
WUSCHEL (WUS) is essential for preventing stem cell differentiation inArabidopsis. Here we report that in addition to its functions in meristematic stem cell maintenance, WUS is involved in the regulation of cell division. TheWUSgain-of-function mutant, stem ectopic flowers (sef), displayed elongated hypocotyls, whereas the loss-of-functionwus-1mutant had shortened hypocotyls. The long hypocotyl insefwas due to the presence of more cells, rather than increased cell elongation. Microscopic observation, flow cytometry assays, quantitative RT-PCR (qRT-PCR), and histochemical staining ofCycB1;1::GUSsupported the hypothesis that ectopic cell division occurred in thesefhypocotyls after germination. Both immunoblot and qRT-PCR results showed thatWUSwas ectopically expressed insefhypocotyls. Luciferase activity, chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) showed thatGLUTAMINE-RICHPROTEIN23(GRP23) expression can be activated by WUS and thatGRP23is a direct target gene of WUS. The phenotypes of35S::GRP23plants andGRP23knockdown lines supported the notion thatGRP23mediates the effects ofWUSon hypocotyl length. Together, our data suggest that ectopic expression ofWUSin hypocotyl controls cell division through its target geneGRP23.
