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The F-box protein OsFBK12 targets OsSAMS1 for degradation and affects pleiotropic phenotypes including leaf senescence in rice

作　　者：Chen Y, Xu YY, Luo W, Li WX, Chen N, Zhang D, Chong K*
影响因子：6.555
刊物名称：Plant Physiology
出版年份：2013
卷：  期：  页码：doi:10.1104/pp.113.224527

论文摘要：

Leaf senescence is related to the grain filling rate and grain weight in cereals. Many components involved in senescence regulation at either the genetic or physiological level are known. However, less is known about molecular regulation mechanisms. Here we report that OsFBK12 (F-box protein containing a kelch repeat motif) interacts with S-adenosyl-l-methionine synthetase1 (SAMS1) to regulate leaf senescence and seed size, as well as grain number in rice. Yeast two-hybrid, pull-down and bimolecular fluorescence complementation assays indicate that OsFBK12 interacts with OSK1 (Oryza sativa SKP1-like protein) and with OsSAMS1. Biochemical and physiological data showed that OsFBK12 targets OsSAMS1 for degradation. OsFBK12-RNAi (FR) lines and OsSAMS1 overexpression (SO) lines showed increased ethylene levels, while OsFBK12-OX (FO) lines and OsSAMS1-RNAi (SR) plants exhibited decreased ethylene. Phenotypically, overexpression of OsFBK12 led to a delay in leaf senescence and germination, and increased seed size, whereas knockdown lines of either OsFBK12 or OsSAMS1 promoted the senescence program. Our results suggest that OsFBK12 is involved in the 26S proteasome pathway by interacting with OSK1 and that it targets the substrate OsSAMS1 for degradation, triggering changes in ethylene levels for regulation of leaf senescence and grain size. These data have potential applications in molecular breeding of rice.
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