A receptor-like protein RMC is involved in regulation of iron acquisition in rice
作 者:Yang A, Li YS, Xu YY, Zhang WH*
影响因子:5.242
刊物名称:Journal of Experimental Botany
出版年份:2013
卷:64 期:16 页码:5009-5020
论文摘要:
Iron (Fe) is one of the essential mineral elements for plant growth and development. Acquisition of Fe by plants is mediated by a complex network involving Fe mobilization, uptake by root cells, and transport within plants. Here, we evaluated the role of a previously clarified gene encoding a receptor-like protein from rice,OsRMC, in the regulation of Fe acquisition by comparing Fe concentration, biomass, and expression patterns of genes associated with Fe mobilization and transport in wild-type (WT) rice with those inOsRMCoverexpression and RNA interference (RNAi) knockdown transgenic rice plants. Expression ofOsRMCwas upregulated in both shoots and roots upon exposure of WT to Fe-deficient medium. Expression levels ofOsRMCwere positively correlated with Fe concentration in rice plants under both Fe-sufficient and Fe-deficient conditions such that overexpression and RNAi lines had higher and lower Fe concentration in both roots and shoots than WT plants, respectively. Moreover, overexpression ofOsRMCconferred greater accumulation of Fe in mature seeds under Fe-sufficient conditions.OsRMCmay also play a role in regulation of Fe deficiency-induced changes in root growth, as evidenced by greater and smaller root systems ofOsRMCoverexpression lines and RNAi lines than WT under Fe-deficient conditions, respectively. Several Fe deficiency-responsive genes includingOsDMAS1,OsNAS1,OsNAS2,OsNAAT1,OsIRT1,OsYSL15, andOsIRO2were up- and downregulated inOsRMC-overexpressing and RNAi plants compared with WT rice plants. These novel findings highlight an important role ofOsRMCplayed in mediation of Fe acquisition and root growth in rice, particularly under Fe-deficient conditions.