Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNA in developing pollen of Oryza sativa
作 者：Wei LQ,Yan LF, Wang T
影响因子：6.885
刊物名称：Genome Biology
出版年份：Jun 2011
卷：12 期：6 页码：R53
论文摘要：
Background Pollen development in flowering plants requires strict control of the gene expression program and genetic information stability by mechanisms possibly including the miRNA pathway. However, our understanding of the miRNA pathway in pollen development remains limited, and the dynamic profile of miRNAs in developing pollen is unknown. Results Using next-generation sequencing technology, we pyrosequenced small RNA populations from rice uninucleate microspores to tricellular pollen and control sporophytic tissues at the genome-wide level. We identified 292 known miRNAs, including members of all 20 plant conserved families, and 75 novel miRNAs. Of the 292 known miRNAs, 202 were expressed, with 103 enriched, in developing pollen. More than half of these novel miRNAs displayed pollen- or stage-specific expression. Furthermore, analyzing the 367 miRNAs and their predicted targets indicated that correlation in expression profiles of pollen-enriched known miRNAs and their targets significantly differs from that of sporophyte-enriched known miRNAs and their targets in some functional terms, while novel miRNAs appeared regulated negatively their targets. Importantly, gene-ontology abundance analysis demonstrated chromatin assembly and disassembly was important in the targets of bicellular pollen-expressed novel miRNAs. Principal component analysis revealed the 3-stages pollen was discriminated from sporophytes, largely because of the novel and non-conserved known miRNAs. Conclusions Our study, for the first time, revealed the differences in composition and expression profile of miRNAs between developing pollen and sporophytes, and novel and non-conserved known miRNAs would be the main contributors. Our results suggested the important roles of the miRNA pathway in pollen development.
