Genome-wide analysis of the LEA (late embryogenesis abundant) protein gene family in Populus trichocarpa
作 者：Ting Lan, Jie Gao, Qing-Yin Zeng
影响因子：2.335
刊物名称：Tree Genetics & Genomes
出版年份：2013
卷：9 期：1 页码：253-264
论文摘要：
Late embryogenesis abundant (LEA) proteins are a large and highly diverse group of polypeptides that are believed to function in desiccation and freezing tolerance in plants. This report presents a genome-wide analysis of LEA proteins and their encoding genes inPopulus trichocarpa. Fifty-threeLEAgenes were identified from thePopulusgenome and divided into eight groups. The LEA4 and LEA5 groups were found in green algae and all land plants, whereas the other six groups existed only in land plants, indicating that theLEAfamily underwent rapid expansion during the early evolution of land plants. A majority ofPopulusLEA proteins contained repeated motifs that were often specific to a LEA group. Except forPtLEA2-1andPtLEA2-3, allPopulusLEA proteins were highly hydrophilic. Examination of the chromosomal locations ofPopulus LEAgenes revealed that 30 % were arranged in tandem repeats, indicating that tandem duplications significantly contributed to the expansion of this gene family inPopulus. Expression patterns of allPopulus LEAgenes under normal growth conditions and abiotic stress (salinity and drought) were investigated by reverse transcription polymerase chain reaction. Twelve of 53Populus LEAgenes were selectively expressed in a specific tissue and/or in response to a specific treatment.LEAgenes also showed extensive divergence in expression patterns, even among those from the same group or gene cluster. The expression profiles revealed that thePopulus LEAgene family was not involved systematically in the same regulatory pathway.
