1. 首页 > 资源库 > 中文 > 植物所论文库

Genome-wide transcriptome and proteome profiles indicate an active role of alternative splicing during de-etiolation of maize seedlings

作　　者：Yan Z, Shen Z, Li Z, Chao Q, Kong L, Gao ZF, Li QW, Zheng HY, Zhao CF, Lu CM, Wang YW*, Wang BC*
影响因子：3.390
刊物名称：Planta
出版年份：2020
卷：252  期：4  页码：60

论文摘要：

The drastic switch from skotomorphogenic to photomorphogenic development is an excellent system to elucidate rapid developmental responses to environmental stimuli in plants. To decipher the effects of different light wavelengths on de-etiolation, we illuminated etiolated maize seedlings with blue, red, blue–red mixed and white light, respectively. We found that blue light alone has the strongest effect on photomorphogenesis and that this effect can be attributed to the higher number and expression levels of photosynthesis and chlorosynthesis proteins. Deep sequencing-based transcriptome analysis revealed gene expression changes under different light treatments and a genome-wide alteration in alternative splicing (AS) profiles. We discovered 41,188 novel transcript isoforms for annotated genes, which increases the percentage of multi-exon genes with AS to 63% in maize. We provide peptide support for all defined types of AS, especially retained introns. Further in silico prediction revealed that 58.2% of retained introns have changes in domains compared with their most similar annotated protein isoform. This suggests that AS acts as a protein function switch allowing rapid light response through the addition or removal of functional domains. The richness of novel transcripts and protein isoforms also demonstrates the potential and importance of integrating proteomics into genome annotation in maize.
全文链接：https://link.springer.com/article/10.1007%2Fs00425-020-03464-5
版权所有 © 中国科学院植物研究所　备案号：京ICP备16067583号-24　文保网安备案号：1101080078
地址：北京市海淀区香山南辛村20号　邮编：100093
电话：010-62590835