Monoterpenoids are key contributors to the characteristic aroma of Muscat-scented grapes, yet their biosynthesis and underlying mechanism to cluster-specific blue light remain poorly understood. In this study, three independent experiments in table Muscat-scented grape demonstrated that blue light is the most effective light quality for promoting monoterpenoid biosynthesis, particularly linalool accumulation. Notably, blue light applied exclusively to the cluster zone significantly enhanced monoterpenoid profiles through a leaf-independent pathway. Furthermore, transcriptome analysis revealed that blue light markedly upregulated genes involved in terpenoid biosynthesis compared with dark-treated controls. Weighted gene co-expression network analysis (WGCNA) identified the MEblue as the module most strongly associated with monoterpenoid contents. Functional enrichment analysis highlighted DXR, LIS, and TPS54 as key structural genes mediating blue light-induced monoterpenoid biosynthesis, while VvbHLH02 and VvTH12 were predicted as potential transcriptional regulators. Together, these results elucidate the regulatory network underlying blue light-induced monoterpenoid biosynthesis in grape berries and provide a molecular basis for improving grape aroma through targeted light management strategies.