Bamboo shoots are prone to browning and microbial infection after harvest, resulting in quality deterioration and yield loss. This study combined carvone-tannic acid-chitosan (CAR-TA-CS) composite film with low temperature storage to alleviate postharvest quality deterioration of bamboo shoots. The results showed that the composite film displayed excellent physic-chemical properties (higher UV-barrier properties and 76.6% reduction in total light transmittance), higher stability and lower water/gas permeability (representing 82.4% and 26.1% reduction, respectively). Further integration of composite film with low temperature storage dramatically reduced weight loss and browning index, while maintaining the antioxidant capacity of bamboo shoots during storage. Moreover, this synergistic regime also completely inhibited the in vitro growth of major fungal pathogens, such as Botrytis cinerea, Apiospora chromolaenae and Talaromyces purpureogenus. Collectively, these results provide a feasible scheme for keeping freshness of bamboo shoots, exhibiting dual advantages of antifungal efficacy and quality maintenance.