1. 首页 > 资源库 > 中文 > 植物所论文库

Identification and evaluation of ω-3 fatty acid desaturase genes for hyper-fortifying α-linolenic acid in transgenic rice seed

作　　者：Hua Liang Liu, Zhi Jie Yin, Li Xiao, Yi Nong Xu, Le Qing Qu
影响因子：4.818
刊物名称：Journal of Experimental Botany
出版年份：2012
卷：63  期：8  页码：3279–3287

论文摘要：

α-Linolenic acid (ALA) deficiency and a skewed of ω6:ω3 fatty acid ratio in the diet are a major explanation for the prevalence of cardiovascular diseases and inflammatory/autoimmune diseases. There is a need to enhance the ALA content and to reduce the ratio of linoleic acid (LA) to ALA. Six ω-3 (△-15) fatty acid desaturase (FAD) genes were cloned from rice and soybean. The subcellular localizations of the proteins were identified. The FAD genes were introduced into rice under the control of an endosperm-specific promoter, GluC, or a Ubi-1 promoter to evaluate their potential in increasing the ALA content in seeds. The ALA contents in the seeds of endoplasmic reticulum (ER)- localized GmFAD3-1 and OsFAD3 overexpression lines increased from 0.36 mg g-1 to 8.57 mg g-1and 10.06 mg g-1, respectively, which was 23.8- and 27.9-fold higher than that of non-transformants. The trait of high ALA content was stably inheritable over three generations. Homologous OsFAD3 is more active than GmFAD3-1 in catalysing LA conversion to ALA in rice seeds. Overexpression of ER-localized GmFAD3-2/3 and chloroplast-localized OsFAD7/8 had less effect on increasing the ALA content in rice seeds. The GluC promoter is advantageous compared with Ubi-1 in this experimental system. The enhanced ALA was preferentially located at the sn-2 position in triacylglycerols. A meal-size portion of high ALA rice would meet >80% of the daily adult ALA requirement. The ALA-rich rice could be expected to ameliorate much of the global dietary ALA deficiency.
全文链接：
版权所有 © 中国科学院植物研究所　备案号：京ICP备16067583号-24　文保网安备案号：1101080078
地址：北京市海淀区香山南辛村20号　邮编：100093
电话：010-62590835