Expression and Localization of Amorpha-4,11-diene Synthase in Artemisia annua L.
作 者：Gao-Bin Pu, Dong-Ming Ma, Hong Wang, He-Chun Ye, Ben-Ye Liu
影响因子：2.453
刊物名称：Plant Molecular Biology Reporter
出版年份：2013
卷：31 期：1 页码：32-37
论文摘要：
Artemisia annuaL. is the only commercial source of the widely used antimalarial compound artemisinin. Biosynthesis of artemisinin has been proposed to take place in glandular trichomes. The first committed step in the conversion of farnesyl diphosphate (FDP) to artemisinin is conducted by the amorpha-4,11-diene synthase (ADS). To explore the organ-specific and developmental distributions of ADS, rabbit polyclonal antibodies were raised against recombinant ADS produced inEscherichia colifrom the correspondingA. annuacDNA. Protein gel blot analysis of differentA. annuaorgans showed that ADS was most abundant in young leaves and flower buds. Minor amounts of ADS were found in mature leaves. These findings were generally consistent with the analysis of the transcript level of theADSgene. Immunolocalization of ADS showed strong positive staining in apical meristems, young leaves and glandular trichomes. No staining was observed in other cells of the leaf. The whole mount hybridization revealed that ADS was not expressed in all glandular trichomes of mature leaves. Specific staining of ADS could be detected in about 10–40 % of glandular trichomes.
