An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake
作 者：He ZY, Yan HL, Chen YS, Shen HL, Xu WX, Zhang HY, Shi L, Zhu YG*, Ma M*
影响因子：7.672
刊物名称：New Phytologist
出版年份：2016
卷：209 期：2 页码：746-761
论文摘要：
The fernPteris vittatais an arsenic hyperaccumulator. The genes involved in arsenite (As(III)) transport are not yet clear. Here, we describe the isolation and characterization of a newP. vittataaquaporin gene,PvTIP4;1, which may mediate As(III) uptake.
PvTIP4;1was identified from yeast functional complement cDNA library ofP. vittata. Arsenic toxicity and accumulating activities ofPvTIP4;1were analyzed inSaccharomyces cerevisiaeandArabidopsis. Subcellular localization of PvTIP4;1–GFP fusion protein inP. vittataprotoplast and callus was conducted. The tissue expression ofPvTIP4;1was investigated by quantitative real-time PCR. Site-directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied.
Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. TransgenicArabidopsisshowed thatPvTIP4;1increases arsenic accumulation and induces arsenic sensitivity. Images and FM4-64 staining suggest that PvTIP4;1 localizes to the plasma membrane inP. vittatacells. A tissue location study shows thatPvTIP4;1transcripts are mainly expressed in roots. Site-directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site.
PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin fromP. vittataand the function and location results imply thatPvTIP4;1may be involved in As(III) uptake.
