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Identification of the target genes of AqAPETALA3-3 (AqAP3-3) in Aquilegia coerulea (Ranunculaceae) helps understand the molecular bases of the conserved and nonconserved features of petals

作　　者：Jiang YC, Wang MM, Zhang R, Xie JH, Duan XS, Shan HY, Xu GX*, Kong HZ*
影响因子：8.512
刊物名称：New Phytologist
出版年份：2020
卷：  期：  页码：DOI: 10.1111/nph.16601

论文摘要：

  • Identification and comparison of the conserved and variable downstream genes of floral organ identity regulators are critical to understanding the mechanisms underlying the commonalities and peculiarities of floral organs. Yet, because of the lack of studies in nonmodel species, a general picture of the regulatory evolution between floral organ identity genes and their targets is still lacking.
  • Here, by conducting extensive chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), electrophoretic mobility shift assay and bioinformatic analyses, we identify and predict the target genes of a petal identity gene, AqAPETALA3-3 (AqAP3-3 ), in Aquilegia coerulea (Ranunculaceae) and compare them with those of its counterpart in Arabidopsis thaliana , AP3 .
  • In total, 7049 direct target genes are identified for AqAP3-3 , of which 2394 are highly confident and 1085 are shared with AP3 . Gene Ontology enrichment analyses further indicate that conserved targets are largely involved in the formation of identity-related features, whereas nonconserved targets are mostly required for the formation of species‐specific features.
  • These results not only help understand the molecular bases of the conserved and nonconserved features of petals, but also pave the way to studying the regulatory evolution between floral organ identity genes and their targets.
全文链接：https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.16601
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