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Characterization of chromatin accessibility and gene expression upon cold stress reveals that the RAV1 transcription factor functions in cold response in Vitis Amurensis

作　　者：Ren C, Li HY, Wang ZM, Dai ZW, Lecourieux F, Kuang YF, Xin HP, Li SH, Liang ZC*
影响因子：5.516
刊物名称：Plant and Cell Physiology
出版年份：2021
卷：62  期：10  页码：1615-1629

论文摘要：

Cold tolerance is regulated by a variety of transcription factors (TFs) and their target genes. Except for the well characterized C-repeat binding factors (CBFs)-dependent transcriptional cascade, the mechanisms of cold tolerance mediated by other transcriptional regulatory networks are still largely unknown. Here, we used the assay for transposase-accessible chromatin with sequencing (ATAC-seq) and RNA-seq to identify cold responsive TFs inVitis amurensis, a grape species with high cold hardiness. Nine TFs, including CBF4, RAV1 and ERF104, were identified after cold treatment. Weighted gene co-expression network analysis (WGCNA) and gene ontology (GO) analysis revealed that these TFs may regulate cold response through different pathways. As a prime candidate TF, overexpression ofVaRAV1in grape cells improved its cold tolerance. The transgenic cells exhibited low electrolyte leakage and malondialdehyde content and high peroxidase activity. Moreover, the TF gene TCP8 and a gene involving in homogalacturonan biosynthesis were found to be regulated by VaRAV1, suggesting that the contribution of VaRAV1 to cold tolerance may be achieved by enhancing the stability of cell membrane and regulating the expression of target genes involved in plant cell wall composition. Our work provides novel insights into plant response to cold stress and demonstrates the utility of ATAC-seq and RNA-seq for the rapid identification of TFs in response to cold stress in grapevine. VaRAV1 may play an important role in adaption to cold stress.

全文链接：https://doi.org/10.1093/pcp/pcab115
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